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| Id: | 27238
| | Autor: | Rojas-Espinosa, Oscar; Camarena-Servin, Veronica; Estrada-Garcia, Iris; Arce-Paredes, Patricia; Wek-Rodriguez, Kendy. | | Título: | Mycobacterium lepraemurium, a well-adapted parasite of macrophages: I. Oxygen metabolites.
| | Fonte: | Int. J. Lepr;66(3):365-373, Sept. 1998. tab, graf.
| | Resumo: | We measured the release of reactive oxygen intermediaries [ROI (hydrogen peroxide and superoxide anion)] by murine peritoneal macrophages challenged in vitro with Mycobacterium lepraemurium (MLM), complement-opsonized yeast, M. bovis BCG, M. phlei, or phorbol myristate acetate (PMA). We found that except for MLM, all of the other materials provoked the release of significant amounts of hydrogen peroxide and superoxide. MLM entered the macrophages without triggering their oxidative metabolism. Pre-infection of macrophages with MLM did not alter these cells' capacity to release the normal amounts of ROI in response to other microorganisms or PMA. Killing of MLM did not revert the macrophages' failure to release ROI upon ingestion of the microorganism, nor were macrophages able to produce these toxic metabolites when pre-incubated in the presence of murine gamma interferon (IFN-gamma). MLM has several attributes that allow it to survive within macrophages: a) it is a nontoxigenic microorganism (it does not harm its host), b) it resists the harsh conditions of the intraphagolysosomal milieu (a property perhaps dependent on its thick lipidic envelope), and c) it penetrates the macrophages without triggering their oxidative response (thus avoiding the generation of the toxic intermediaries of oxygen). For these attributes (and others discussed in this paper), we recognize MLM as a highly evolved, well-adapted parasite of macrophages. In addition, the results of the present study prompted the analysis of the biochemical pathways used by MLM and M. bovis BCG to penetrate into their cellular hosts, a subject now under investigation in our laboratory. (AU)^ien.
| | Descritores: | Macrófagos Peritoneais/ef drogas
Macrófagos Peritoneais/metab
Macrófagos Peritoneais/microbiol
Mycobacterium lepraemurium/fisiol
| | Meio Eletrônico: | http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1998/pdf/v66n3/v66n3a07.pdf - en.
| | Localização: | BR191.1 |
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